Occurrence of a common epitope in circumsporozoite proteins of Plasmodium falciparum isolated from different areas in Thailand

Southeast Asian J Trop Med Public Health. 1986 Mar;17(1):13-8.

Abstract

Fifteen isolates of P. falciparum sporozoites obtained from patients with acute falciparum malaria from various malaria endemic areas in Thailand were tested for the presence of a common antigenic determinant in their CS protein molecules. SDS-PAGE and Western blot analysis using MAB or human serum antibodies specific to the CS proteins of the parasites revealed a common epitope shared in the CS proteins of all strains of P. falciparum tested. However, the CS proteins exhibited M.W. variation when different strains of the parasites were compared. A similar result was obtained when the human serum antibodies were used. The present study clearly indicated the occurrence of the common epitope in phenotypically different CS proteins among isolates of P. falciparum sporozoites and supported the notion that antigens containing these repetitive epitopes could be used as the candidates for the sporozoite vaccine against P. falciparum infection.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antigens, Protozoan / analysis*
  • Autoradiography
  • Cross Reactions
  • Electrophoresis, Polyacrylamide Gel
  • Epitopes / analysis*
  • Humans
  • Immunologic Techniques
  • Malaria / immunology
  • Plasmodium falciparum / immunology*
  • Proteins / immunology
  • Thailand

Substances

  • Antigens, Protozoan
  • Epitopes
  • Proteins