Effect of thimerosal on the neurodevelopment of premature rats

World J Pediatr. 2013 Nov;9(4):356-60. doi: 10.1007/s12519-013-0443-z. Epub 2013 Nov 14.

Abstract

Background: This study was undertaken to determine the effect of thimerosal on the neurodevelopment of premature rats.

Methods: Thimerosal was injected into premature SD rats at a dose of 32.8, 65.6, 98.4 or 131.2 μg/kg on postnatal day 1. Expression of dopamine D4 receptor (DRD4) and serotonin 2A receptor (5-HT2AR), apoptosis in the prefrontal cortex on post-injection day 49, and learning and memory function were studied and compared with those in a control group injected with saline.

Results: Expression of DRD4 and 5-HT2AR and learning function decreased, and apoptosis increased significantly in the 131.2 μg/kg group (P<0.001). Memory function was significantly impaired by 65.6 (P<0.05), 98.4 and 131.2 μg/kg (P<0.001).

Conclusions: The negative adverse consequences on neurodevelopment observed in the present study are consistent with previous studies; this study raised serious concerns about adverse neurodevelopmental disorder such as autism in humans following the ongoing worldwide routine administration of thimerosalcontaining vaccines to infants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Animals, Newborn
  • Apoptosis / drug effects
  • Immunohistochemistry
  • Injections
  • Maze Learning / drug effects*
  • Memory / drug effects*
  • Prefrontal Cortex / metabolism
  • Preservatives, Pharmaceutical / administration & dosage
  • Preservatives, Pharmaceutical / adverse effects*
  • Rats
  • Rats, Sprague-Dawley
  • Receptor, Serotonin, 5-HT2A / metabolism
  • Receptors, Dopamine D4 / metabolism
  • Thimerosal / administration & dosage
  • Thimerosal / adverse effects*

Substances

  • Drd4 protein, rat
  • Preservatives, Pharmaceutical
  • Receptor, Serotonin, 5-HT2A
  • Receptors, Dopamine D4
  • Thimerosal