Optimization of steady-state ¹³C-labeling experiments for metabolic flux analysis

Methods Mol Biol. 2014:1090:53-72. doi: 10.1007/978-1-62703-688-7_4.

Abstract

While steady-state (13)C metabolic flux analysis is a powerful method for deducing multiple fluxes in the central metabolic network of heterotrophic and mixotrophic plant tissues, it is also time-consuming and technically challenging. Key steps in the design and interpretation of steady-state (13)C labeling experiments are illustrated with a generic protocol based on applications to plant cell suspension cultures.

MeSH terms

  • Algorithms
  • Arabidopsis / cytology
  • Arabidopsis / metabolism
  • Carbohydrate Metabolism
  • Carbon Isotopes / metabolism
  • Cell Culture Techniques
  • Cells, Cultured
  • Computer Simulation
  • Kinetics
  • Metabolic Flux Analysis*
  • Metabolic Networks and Pathways
  • Models, Biological
  • Monte Carlo Method
  • Plant Cells / metabolism
  • Staining and Labeling

Substances

  • Carbon Isotopes