In vitro selection of peptide aptamers using a ribosome display for a conducting polymer

J Biosci Bioeng. 2014 Apr;117(4):501-3. doi: 10.1016/j.jbiosc.2013.10.005. Epub 2013 Nov 5.

Abstract

Ribosome display was used to select peptide aptamers from a random library composed of hydrophilic amino acids for a conducting polymer, poly(3-hexylthiophene-2,5-diyl). Binding of aptamers was measured by quartz crystal microbalance, and the secondary structure of the peptide was investigated by circular dichroism.

Keywords: Binding ability; In vitro selection; Peptide aptamer; Poly(3-hexylthiophene-2,5-diyl); Ribosome display.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Peptide / chemistry
  • Aptamers, Peptide / isolation & purification*
  • Aptamers, Peptide / metabolism*
  • Circular Dichroism
  • Electric Conductivity*
  • Peptide Library*
  • Polymers / chemistry*
  • Polymers / metabolism*
  • Protein Binding
  • Quartz Crystal Microbalance Techniques
  • Ribosomes / metabolism*
  • Semiconductors
  • Thiophenes / chemistry*
  • Thiophenes / metabolism*

Substances

  • Aptamers, Peptide
  • Peptide Library
  • Polymers
  • Thiophenes
  • poly(3-hexylthiophene-2,5-diyl)