The effect of anions on K(+)-binding in aHalobacterium species

M Ginzburg; B Z Ginzburg; D C Tosteson

doi:10.1007/BF01872280

The effect of anions on K(+)-binding in aHalobacterium species

J Membr Biol. 1971 Sep;6(3):259-68. doi: 10.1007/BF01872280.

Authors

M Ginzburg¹, B Z Ginzburg, D C Tosteson

Affiliation

¹ Department of Physiology and Pharmacology, Duke University Medical Center, 27706, Durham, North Carolina.

PMID: 24177387
DOI: 10.1007/BF01872280

Abstract

Trinitrocresolate (TNC) at a concentration of 2×10(-3) M brings about rapid loss of K from starvingHalobacterium cells. Higher concentrations of other anions such as salicylate, thiocyanate, and perchlorate produce a similar effect. The TNC-induced K loss is not significantly reversed when TNC is removed from the ambient medium. The rate of K loss in the presence of 2×10(-3) M TNC is only slightly increased by the temperature in the ranges of 30 to 40°C and 0 to 20°C; between 20 and 30°C, however, the rate increases 10-fold. The K loss was partly replaced by Na(+). These data are interpreted in terms of the hypothesis that K is retained in starvingHalobacterium sp. not by active transport, but rather by selective binding on loci which are modified by TNC.