Effect of methylprednisolone and edaravone administration on spinal cord injury

Eur Rev Med Pharmacol Sci. 2013 Oct;17(20):2766-72.

Abstract

Background: Spinal cord injury (SCI) is one of the most devastating traumatic conditions that primarily affects young males with an annual incidence of 15-40 cases per million.

Aim: To explore the superior neuroprotective effect of edaravone (ED) on spinal cord injury during maintenance therapy compared with methylprednisolone (MP).

Materials and methods: Sprague-Dawley rat model of spinal cord injury was established by modified Allen's method. Total 114 rats were divided into two groups and then six subgroups individually: A1 (control group, normal saline injection within 8 h), B1 (MP group, MP injection within 8 h), C1(ED group, ED injection within 8 h), A2 (control group, normal saline injection after 8 h), B1 (MP group, MP injection after 8 h), C1 (ED group, ED injection after 8 h). Further, we investigated the changes of histopathology, caspase-3 and Bcl-xL positive cell.

Results: Haemorrhage, swelling, hyperaemia, gliocytes hyperplasia, inflammatory cells infiltration, vacuolar denaturation, and nucleus concentration could be observed, especially in control group. Caspase-3 positive cell was significantly decreased in MP and ED group within 8 h administration, but caspase-3 positive cell was only significantly decreased in ED group after 8 h administration. And B-cell lymphoma extra large (Bcl-xL) was significantly increased in ED group than MP group no matter within 8 h or after 8 h administration.

Conclusions: More attention should be paid on the time point of MP administration, and ED administration seem to be more effective for maintenance therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antipyrine / analogs & derivatives*
  • Antipyrine / therapeutic use
  • Caspase 3 / analysis
  • Edaravone
  • Methylprednisolone / therapeutic use*
  • Rats
  • Rats, Sprague-Dawley
  • Spinal Cord Injuries / drug therapy*
  • Spinal Cord Injuries / pathology
  • bcl-X Protein / analysis

Substances

  • Bcl2l1 protein, rat
  • bcl-X Protein
  • Caspase 3
  • Edaravone
  • Antipyrine
  • Methylprednisolone