Multiplex Real-Time Polymerase Chain Reaction-Based Method for the Rapid Detection of gyrA and parC Mutations in Quinolone-Resistant Escherichia coli and Shigella spp

Junyoung Kim; Semi Jeon; Hyungjun Kim; Misun Park; Soobok Kim; Seonghan Kim

doi:10.1016/j.phrp.2012.04.004

Multiplex Real-Time Polymerase Chain Reaction-Based Method for the Rapid Detection of gyrA and parC Mutations in Quinolone-Resistant Escherichia coli and Shigella spp

Osong Public Health Res Perspect. 2012 Jun;3(2):113-7. doi: 10.1016/j.phrp.2012.04.004.

Authors

Junyoung Kim¹, Semi Jeon, Hyungjun Kim, Misun Park, Soobok Kim, Seonghan Kim

Affiliation

¹ Division of Enteric Bacterial Infections, Korea National Institute of Health, Osong, Korea.

Abstract

Two real-time polymerase chain reaction assays were developed to detect mutations in codons 83 and 87 in gyrA and in codons 80 and 91 in parC, the main sites that causes quinolone resistance in pathogenic Escherichia coli and Shigella spp. isolates. These assays can be employed as a useful method for controlling infections caused by quinolone-resistant E coli and Shigella isolates.

Keywords: fluoroquinolone; gyrA; multiplex real-time PCR; parC.