Abstract
The quiescent center (QC) plays an essential role during root development by creating a microenvironment that preserves the stem cell fate of its surrounding cells. Despite being surrounded by highly mitotic active cells, QC cells self-renew at a low proliferation rate. Here, we identified the ERF115 transcription factor as a rate-limiting factor of QC cell division, acting as a transcriptional activator of the phytosulfokine PSK5 peptide hormone. ERF115 marks QC cell division but is restrained through proteolysis by the APC/C(CCS52A2) ubiquitin ligase, whereas QC proliferation is driven by brassinosteroid-dependent ERF115 expression. Together, these two antagonistic mechanisms delimit ERF115 activity, which is called upon when surrounding stem cells are damaged, revealing a cell cycle regulatory mechanism accounting for stem cell niche longevity.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Anaphase-Promoting Complex-Cyclosome / metabolism
-
Arabidopsis / cytology*
-
Arabidopsis / growth & development*
-
Arabidopsis Proteins / genetics
-
Arabidopsis Proteins / metabolism*
-
Cell Cycle / genetics
-
Cell Cycle / physiology
-
Cell Cycle Proteins / metabolism
-
Cell Division / genetics
-
Cell Division / physiology*
-
Mitosis / genetics
-
Mitosis / physiology
-
Peptide Hormones / genetics
-
Peptide Hormones / metabolism
-
Plant Roots / cytology*
-
Plant Roots / growth & development*
-
Proteolysis
-
Signal Transduction
-
Stem Cell Niche
-
Stem Cells / physiology*
-
Transcription Factors / genetics
-
Transcription Factors / metabolism*
Substances
-
Arabidopsis Proteins
-
CCS52A2 protein, Arabidopsis
-
Cell Cycle Proteins
-
ERF115 protein, Arabidopsis
-
PSK5 protein, Arabidopsis
-
Peptide Hormones
-
Transcription Factors
-
Anaphase-Promoting Complex-Cyclosome
Associated data
-
GEO/GSE48793
-
GEO/GSE48836