Thermal responsive microgels as recyclable carriers to immobilize active proteins with enhanced nonaqueous biocatalytic performance

Chem Commun (Camb). 2013 Dec 14;49(96):11299-301. doi: 10.1039/c3cc46161k. Epub 2013 Oct 24.

Abstract

We describe the preparation of a thermoresponsive microgel, which can non-covalently immobilize active proteins with enhanced biocatalytic performance in organic solvents and easy reusability due to the porous microstructure and temperature responsive property.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylamides / chemistry
  • Animals
  • Biocatalysis
  • Gels / chemistry*
  • Hemoglobins / chemistry*
  • Horseradish Peroxidase / chemistry
  • Hydrogen Peroxide / chemistry
  • Hydrogen-Ion Concentration
  • Immobilized Proteins / chemistry*
  • Models, Molecular
  • Oxidation-Reduction
  • Porosity
  • Temperature

Substances

  • Acrylamides
  • Gels
  • Hemoglobins
  • Immobilized Proteins
  • N-isopropylacrylamide
  • Hydrogen Peroxide
  • Horseradish Peroxidase