Abstract
Next-generation DNA sequencing can be used to catalog individual organisms within complex, polymicrobial specimens. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and conventional molecular methods were overwhelmed by overgrowth of other organisms.
MeSH terms
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Actinomycetales / classification
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Actinomycetales / genetics
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Actinomycetales / isolation & purification*
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Cluster Analysis
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics
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Diabetes Complications
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Female
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Foot / pathology
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High-Throughput Nucleotide Sequencing
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Histocytochemistry
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Humans
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Microscopy
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Middle Aged
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Molecular Sequence Data
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Mycetoma / diagnosis*
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Mycetoma / microbiology
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Phylogeny
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RNA, Ribosomal, 16S / genetics
Substances
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DNA, Bacterial
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DNA, Ribosomal
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RNA, Ribosomal, 16S