Molecular diagnosis of Actinomadura madurae infection by 16S rRNA deep sequencing

J Clin Microbiol. 2013 Dec;51(12):4262-5. doi: 10.1128/JCM.02227-13. Epub 2013 Oct 9.

Abstract

Next-generation DNA sequencing can be used to catalog individual organisms within complex, polymicrobial specimens. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and conventional molecular methods were overwhelmed by overgrowth of other organisms.

Publication types

  • Case Reports

MeSH terms

  • Actinomycetales / classification
  • Actinomycetales / genetics
  • Actinomycetales / isolation & purification*
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Diabetes Complications
  • Female
  • Foot / pathology
  • High-Throughput Nucleotide Sequencing
  • Histocytochemistry
  • Humans
  • Microscopy
  • Middle Aged
  • Molecular Sequence Data
  • Mycetoma / diagnosis*
  • Mycetoma / microbiology
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/KF680773