Increased IL-33 in synovial fluid and paired serum is associated with disease activity and autoantibodies in rheumatoid arthritis

Clin Dev Immunol. 2013:2013:985301. doi: 10.1155/2013/985301. Epub 2013 Sep 9.

Abstract

Objectives: IL-33, a newly found cytokine which is involved in joint inflammation, could be blocked by a decoy receptor-sST2. The expression and correlation of IL-33 and sST2 in rheumatoid arthritis (RA) are of great interest.

Methods: Synovial fluid (SF) was obtained from 120 RA and 30 osteoarthritis (OA) patients, and paired sera were collected from 54 of these RA patients. The levels of IL-33 and sST2 were measured by ELISA.

Results: SF IL-33 was significantly higher in RA than in OA, which was correlated with disease activity score 28, erythrocyte sedimentation rate, rheumatoid factor (RF)-IgM, RF-IgG, glucose phosphate isomerase (GPI), and immunoglobulin. Serum IL-33 was correlated positively with SF IL-33 in RA. Furthermore, it was correlated with RF-IgM and GPI. sST2 was partly detectable in RA (13 out of 54, 24.1%), while not in OA. Serum sST2 in RA had no significant correlation with serum IL-33 or SF IL-33. However, SFs from both RA and OA patients did not express sST2.

Conclusions: This study supported that IL-33 played an important role in the local pathogenesis of RA. Considering the tight correlation between IL-33 and clinical features, it may become a new target of local treatment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Arthritis, Rheumatoid / diagnosis
  • Arthritis, Rheumatoid / immunology*
  • Arthritis, Rheumatoid / metabolism*
  • Autoantibodies / blood
  • Autoantibodies / immunology*
  • Biomarkers
  • Child
  • Female
  • Humans
  • Interleukin-33
  • Interleukins / blood
  • Interleukins / metabolism*
  • Male
  • Middle Aged
  • Osteoarthritis / diagnosis
  • Osteoarthritis / immunology
  • Osteoarthritis / metabolism
  • Synovial Fluid / immunology
  • Synovial Fluid / metabolism
  • Young Adult

Substances

  • Autoantibodies
  • Biomarkers
  • IL33 protein, human
  • Interleukin-33
  • Interleukins