Copy number variants are frequent in genetic generalized epilepsy with intellectual disability

Neurology. 2013 Oct 22;81(17):1507-14. doi: 10.1212/WNL.0b013e3182a95829. Epub 2013 Sep 25.

Abstract

Objective: We examined whether copy number variants (CNVs) were more common in those with a combination of intellectual disability (ID) and genetic generalized epilepsy (GGE) than in those with either phenotype alone via a case-control study.

Methods: CNVs contribute to the genetics of multiple neurodevelopmental disorders with complex inheritance, including GGE and ID. Three hundred fifty-nine probands with GGE and 60 probands with ID-GGE were screened for GGE-associated recurrent microdeletions at 15q13.3, 15q11.2, and 16p13.11 via quantitative PCR or loss of heterozygosity. Deletions were confirmed by comparative genomic hybridization (CGH). ID-GGE probands also had genome-wide CGH.

Results: ID-GGE probands showed a significantly higher rate of CNVs compared with probands with GGE alone, with 17 of 60 (28%) ID-GGE probands having one or more potentially causative CNVs. The patients with ID-GGE had a 3-fold-higher rate of the 3 GGE-associated recurrent microdeletions than probands with GGE alone (10% vs 3%, p = 0.02). They also showed a high rate (13/60, 22%) of rare CNVs identified using genome-wide CGH.

Conclusions: This study shows that CNVs are common in those with ID-GGE with recurrent deletions at 15q13.3, 15q11.2, and 16p13.11, particularly enriched compared with individuals with GGE or ID alone. Recurrent CNVs are likely to act as risk factors for multiple phenotypes not just at the population level, but also in any given individual. Testing for CNVs in ID-GGE will have a high diagnostic yield in a clinical setting and will inform genetic counseling.

Publication types

  • Case Reports
  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Case-Control Studies
  • Child
  • Chromosome Aberrations
  • Chromosome Deletion
  • Chromosome Disorders / genetics
  • Chromosomes, Human, Pair 15 / genetics
  • Chromosomes, Human, Pair 16 / genetics
  • Cohort Studies
  • Comorbidity
  • DNA Copy Number Variations / genetics*
  • Epilepsy, Generalized / epidemiology
  • Epilepsy, Generalized / genetics*
  • Female
  • Genetic Testing
  • Humans
  • Intellectual Disability / epidemiology
  • Intellectual Disability / genetics*
  • Male
  • Middle Aged
  • Phenotype
  • Rubinstein-Taybi Syndrome / genetics
  • Seizures / genetics
  • Young Adult

Supplementary concepts

  • Chromosome 15q13.3 Microdeletion Syndrome
  • Chromosome 16p13.3 Deletion Syndrome
  • Duplication 15q11-q13 Syndrome