Abstract
We present a seminested PCR method that specifically discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. The test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra, which also permits discrimination of intraspecific sequence variants at this locus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA, Protozoan / chemistry
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DNA, Protozoan / genetics*
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Genes, Protozoan
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Genetic Markers*
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Molecular Sequence Data
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Parasitology / methods*
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Plasmodium ovale / classification*
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Plasmodium ovale / genetics*
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Polymerase Chain Reaction / methods*
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Sequence Analysis, DNA
Substances
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DNA, Protozoan
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Genetic Markers
Associated data
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GENBANK/KF018430
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GENBANK/KF018431
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GENBANK/KF018432
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GENBANK/KF018433