Crystallization and preliminary X-ray investigation of recombinant Lactobacillus leichmannii nucleoside deoxyribosyltransferase

J Biol Chem. 1990 Feb 15;265(5):2682-3.

Abstract

Crystals of recombinant bacterial nucleoside deoxyribosyltransferase have been grown from solutions of ammonium sulfate. The crystals are cubic, space group I23 or I2(1)3; the axial length is 151.1(2) A. The crystals are stable to x-rays for at least 5 days and diffract beyond 2.8-A resolution. It appears that the molecule, which is a hexamer, utilizes the symmetry of the space group, resulting in two or three subunits per asymmetric unit.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromatography, Gel
  • Crystallization
  • Escherichia coli / genetics
  • Genes, Bacterial
  • Lactobacillus / enzymology*
  • Lactobacillus / genetics
  • Macromolecular Substances
  • Pentosyltransferases* / genetics
  • Pentosyltransferases* / isolation & purification
  • Protein Conformation
  • Recombinant Proteins / isolation & purification
  • X-Ray Diffraction

Substances

  • Macromolecular Substances
  • Recombinant Proteins
  • Pentosyltransferases
  • nucleoside deoxyribosyltransferase