PNA-FISH assays for early targeted bacteraemia treatment

B J Parcell; G V Orange

doi:10.1016/j.mimet.2013.09.004

PNA-FISH assays for early targeted bacteraemia treatment

J Microbiol Methods. 2013 Nov;95(2):253-5. doi: 10.1016/j.mimet.2013.09.004. Epub 2013 Sep 17.

Authors

B J Parcell¹, G V Orange

Affiliation

¹ Department of Medical Microbiology, Ninewells Hospital and Medical School, NHS Tayside, Dundee, United Kingdom. Electronic address: b.parcell@nhs.net.

PMID: 24055387
DOI: 10.1016/j.mimet.2013.09.004

Abstract

PNA-FISH S. aureus/CNS and GNR Traffic Light assays were compared with standard culture methods for identifying bacteraemia in 156 blood cultures from 131 patients. Results correlated with final culture results in 153 cultures. Retrospective case note review revealed that earlier targeted treatment would have occurred in 10.7% of cases.

Keywords: API; Analytical profile index; Bacteraemia; Blood cultures; CLSI; Clinical and Laboratory Standards Institute; GNR; GPC; Gram negative rods; Gram positive cocci; MALDI-TOF MS; MRSA; MSSA; Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry; Meticillin-resistant Staphylococcus aureus; Meticillin-sensitive Staphylococcus aureus; PCR; PNA-FISH; Peptide nucleic acid fluorescence in situ hybridization; Polymerase chain reaction; Treatment.

MeSH terms

Bacteremia / diagnosis*
Bacteremia / therapy
Escherichia coli / isolation & purification
Humans
In Situ Hybridization, Fluorescence / methods*
Klebsiella pneumoniae / isolation & purification
Peptide Nucleic Acids*
Pseudomonas aeruginosa / isolation & purification
Retrospective Studies
Sensitivity and Specificity
Staphylococcus aureus / isolation & purification*

Substances

Peptide Nucleic Acids