Objective: The aim of this study was to investigate effects of mechanical compressive force on differentiation of adipose-derived stem cells (ASCs) in vitro.
Materials and methods: Mice ASCs were treated with compressive force (2000 με, 1 Hz) for 2 or 6 h after adipogenic induction for 3 days, then oil red O staining was used to examine oil droplet-filled cells. Adipogenic genes, PPAR-γ1 and APN, were examined by real-time PCR and immunofluorescence (IF) staining was performed to test expression of de-PPAR-γ and ph-PPAR-γ at the protein level.
Results: Our data showed that mechanical compressive force reduced numbers of oil droplet-filled cells, and down-regulated mRNA levels of both PPAR-γ1 and APN and protein level of PPAR-γ, in ASCs.
Conclusions: In culture medium containing adipogenic stimuli, mechanical compressive force inhibited adipogenesis of ASCs.
© 2013 John Wiley & Sons Ltd.