Objective: To investigate the serotype virulence genes and molecular typing characteristics of Vibrio Parahaemolyticus isolated from diarrhea samples among 2007 -2012.
Methods: The serotype was detected by using serological agglutination test. Thermo-stable direct hemolysin (tdh) and tdh-related hemolysin (trh) were detected by using real time PCR methods. 56 strains 03:K6 were analyzed for molecular typing by pulsed field gel electrophoresis(PFGE).
Results: (1) There are 14 serotypes, including 03: K6 (114,67.9%),04: K8 (25,14.9%), 03: K29 (6,3.6%) and et al. Compared with the serotypes of strains from food poison, these from sporadic cases with diarrhea were different, but the main serotype was also 03:K6. (2) There are 4 types of virulence genes, including tdh+ trh- (161, 95.8%), tdh+ trh+ (3,1.8%), tdh- trh+ (1, 0.6%) tdh- trh- (3, 1.8%). Compared with the virulence genes of strains from food poison, these from sporadic cases with diarrhea were different, but the main virulence gene was also tdh+ trh-. 3,56 strains belonged to 15 PFGE strips and the similar degree is higher than 86%. 10 patterns (P1-P4, P6, P7, P9, P10, P12, P15) had at lest two strains.
Conclusion: The most V. Parahaemolyticus isolated from clinical patients was 03: K6 and tdh+ trh-. PFGE strips of V. Parahaemolyticus of 03: K6 from clinical patients exhibited a high degree of similarity, and correlation was found among strains.