Adipose tissue NK cells manifest an activated phenotype in human obesity

Metabolism. 2013 Nov;62(11):1557-61. doi: 10.1016/j.metabol.2013.07.011. Epub 2013 Sep 5.

Abstract

Objective: Adipose tissue inflammation is a cause of obesity-related metabolic disease. Natural killer (NK) cells are an understudied cell type in the context of obesity. The goal of this study was to determine the phenotype of human adipose tissue NK cells.

Methods: We used flow cytometry phenotyping to study adipose tissue and peripheral blood NK cells from obese and lean humans.

Results: Human adipose tissue NK cells, relative to peripheral blood NK cells, express increased levels of activation markers. Adipose tissue NK cells also demonstrate an activated phenotype in obese relative to lean subjects, with increased expression of the activating receptor NKG2D.

Conclusions: These data are the first detailed phenotypic characterization of human adipose tissue NK cells, and suggest a role for NK cells in adipose tissue inflammation in obesity.

Keywords: ATM; ATNK; CD56; Inflammation; NKG2D; PB; PBMC; PBNK; SAT; SVF; VAT; adipose tissue NK cells; adipose tissue macrophages; peripheral blood; peripheral blood NK cells; peripheral blood mononuclear cells; stromovascular cell fraction; subcutaneous adipose tissue; visceral adipose tissue.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adipose Tissue / metabolism*
  • Adipose Tissue / pathology*
  • Adult
  • Body Mass Index
  • CD3 Complex / metabolism
  • CD56 Antigen / metabolism
  • Cell Separation
  • Female
  • Flow Cytometry
  • GPI-Linked Proteins / metabolism
  • Humans
  • Inflammation / metabolism
  • Inflammation / pathology
  • Intra-Abdominal Fat / metabolism
  • Intra-Abdominal Fat / pathology
  • Killer Cells, Natural / metabolism*
  • Killer Cells, Natural / pathology*
  • Leukocyte Common Antigens / metabolism
  • Male
  • Middle Aged
  • NK Cell Lectin-Like Receptor Subfamily K / genetics
  • NK Cell Lectin-Like Receptor Subfamily K / metabolism*
  • Obesity / metabolism
  • Obesity / pathology*
  • Phenotype
  • Receptors, IgG / metabolism
  • Subcutaneous Fat / metabolism
  • Subcutaneous Fat / pathology
  • Thinness / metabolism
  • Thinness / pathology
  • Tumor Necrosis Factor Receptor Superfamily, Member 7 / metabolism

Substances

  • CD3 Complex
  • CD56 Antigen
  • FCGR3B protein, human
  • GPI-Linked Proteins
  • KLRK1 protein, human
  • NCAM1 protein, human
  • NK Cell Lectin-Like Receptor Subfamily K
  • Receptors, IgG
  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • Leukocyte Common Antigens
  • PTPRC protein, human