A polyclonal antibody to goldfish neuronal 145 kDa intermediate filament protein

Brain Res. 1990 Jul 30;524(1):133-8. doi: 10.1016/0006-8993(90)90501-2.

Abstract

A polyclonal antibody raised to a protein from goldfish optic tectum recognises, immunohistochemically, axons throughout normal goldfish visual pathway. In goldfish with injured optic nerve, this antibody recognises degenerating neuronal debris as well as regenerating fibres. On immunoblot, the antibody recognises, primarily, a neuronal intermediate filament protein in the region of 145 kDa. Such an antibody should prove useful in studies pertaining to goldfish visual pathway.

MeSH terms

  • Animals
  • Antibodies
  • Electrophoresis, Polyacrylamide Gel
  • Goldfish
  • Immunoblotting
  • Immunohistochemistry
  • Intermediate Filament Proteins / analysis*
  • Intermediate Filament Proteins / immunology
  • Molecular Weight
  • Nerve Fibers / ultrastructure
  • Retina / ultrastructure*
  • Superior Colliculi / ultrastructure*

Substances

  • Antibodies
  • Intermediate Filament Proteins