MiR-215 modulates gastric cancer cell proliferation by targeting RB1

Cancer Lett. 2014 Jan 1;342(1):27-35. doi: 10.1016/j.canlet.2013.08.033. Epub 2013 Aug 24.

Abstract

Growing evidence indicates that miRNAs play critical roles in tumorigenesis and cancer progression. Here, we report that miR-215 is significantly up-regulated in gastric cancer tissues from either gastrectomy or gastroscopy. Receiver Operator Characteristic (ROC) curve analysis indicated that miR-215 may be a candidate biomarker for gastric cancer diagnosis. Inhibition of miR-215 significantly suppressed gastric cancer cell proliferation possibly via G1 arrest. Further analyses indicated that miR-215 was able to target retinoblastoma tumor-suppressor gene 1 (RB1) through its 3'-UTR in gastric cancer cells. These data suggest that frequently up-regulated miR-215 in gastric cancer may influence cell proliferation by targeting RB1.

Keywords: Gastric cancer; MiR-215; Proliferation; Retinoblastoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Aged
  • Area Under Curve
  • Base Sequence
  • Binding Sites
  • Biomarkers, Tumor / genetics*
  • Biomarkers, Tumor / metabolism
  • Cell Cycle
  • Cell Line, Tumor
  • Cell Proliferation*
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Male
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • RNA Interference
  • ROC Curve
  • Retinoblastoma Protein / genetics*
  • Retinoblastoma Protein / metabolism
  • Stomach Neoplasms / diagnosis
  • Stomach Neoplasms / genetics
  • Stomach Neoplasms / metabolism*
  • Transcriptome
  • Up-Regulation

Substances

  • 3' Untranslated Regions
  • Biomarkers, Tumor
  • MIRN215 microRNA, human
  • MicroRNAs
  • Retinoblastoma Protein