DMSO enhances electrospray response, boosting sensitivity of proteomic experiments

Hannes Hahne; Fiona Pachl; Benjamin Ruprecht; Stefan K Maier; Susan Klaeger; Dominic Helm; Guillaume Médard; Matthias Wilm; Simone Lemeer; Bernhard Kuster

doi:10.1038/nmeth.2610

DMSO enhances electrospray response, boosting sensitivity of proteomic experiments

Nat Methods. 2013 Oct;10(10):989-91. doi: 10.1038/nmeth.2610. Epub 2013 Aug 25.

Authors

Hannes Hahne¹, Fiona Pachl, Benjamin Ruprecht, Stefan K Maier, Susan Klaeger, Dominic Helm, Guillaume Médard, Matthias Wilm, Simone Lemeer, Bernhard Kuster

Affiliation

¹ Chair of Proteomics and Bioanalytics, Center of Life and Food Sciences Weihenstephan, Technische Universität München, Freising, Germany.

PMID: 23975139
DOI: 10.1038/nmeth.2610

Abstract

We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost.

MeSH terms

Dimethyl Sulfoxide / chemistry*
Peptides / analysis*
Peptides / chemistry
Proteomics / methods*
Proteomics / standards
Sensitivity and Specificity
Solvents / chemistry*
Spectrometry, Mass, Electrospray Ionization / methods*
Spectrometry, Mass, Electrospray Ionization / standards
Tandem Mass Spectrometry / methods*
Tandem Mass Spectrometry / standards

Substances

Peptides
Solvents
Dimethyl Sulfoxide