The distinct expression patterns of claudin-2, -6, and -11 between human gastric neoplasms and adjacent non-neoplastic tissues

Diagn Pathol. 2013 Aug 6:8:133. doi: 10.1186/1746-1596-8-133.

Abstract

Background: Cancers have a multifactorial etiology a part of which is genetic. Recent data indicate that expression of the tight junction claudin proteins is involved in the etiology and progression of cancer.

Methods: To explore the correlations of the tight junction proteins claudin-2,-6, and -11 in the pathogenesis and clinical behavior of gastric cancer, 40 gastric cancer tissues and 28 samples of non-neoplastic tissues adjacent to the tumors were examined for expression of claudin-2,-6, and -11 by streptavidin-perosidase immunohistochemical staining method.

Results: The positive expression rates of claudin-2 in gastric cancer tissues and adjacent tissues were 25% and 68% respectively (P<0.001). The positive expression rates of claudin-6 in gastric cancer tissues and adjacent tissues were 55% and 79% respectively (P=0.045<0.05). In contrast, the positive expression rates of claudin-11 in gastric cancer tissues and gastric cancer adjacent tissues were 80% and 46% (P=0.004<0.01). Thus in our study, the expression of claudin-2, and claudin-6 was down regulated in gastric cancer tissue while the expression of claudin-11 was up regulated. Correlations between claudin expression and clinical behavior were not observed.

Conclusion: Our study provides the first evidence that claudin-2,-6, and -11 protein expression varies between human gastric cancers and adjacent non-neoplastic tissues.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/5470513569630744.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / analysis*
  • Carcinoma / chemistry*
  • Carcinoma / secondary
  • Claudins / analysis*
  • Female
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Predictive Value of Tests
  • Prognosis
  • Stomach Neoplasms / chemistry*
  • Stomach Neoplasms / pathology

Substances

  • Biomarkers, Tumor
  • CLDN11 protein, human
  • CLDN2 protein, human
  • Claudins
  • claudin 6