Knockdown of the placental growth factor gene inhibits laser induced choroidal neovascularization in a murine model

Ramin Nourinia; Zahra-Soheila Soheili; Hamid Ahmadieh; Hassan Akrami; Mozhgan Rezaei Kanavi; Shahram Samiei

Knockdown of the placental growth factor gene inhibits laser induced choroidal neovascularization in a murine model

J Ophthalmic Vis Res. 2013 Jan;8(1):4-8.

Authors

Ramin Nourinia¹, Zahra-Soheila Soheili, Hamid Ahmadieh, Hassan Akrami, Mozhgan Rezaei Kanavi, Shahram Samiei

Affiliation

¹ Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

PMID: 23825706
PMCID: PMC3691981

Abstract

Purpose: To evaluate the effect of placental growth factor (PlGF) gene knockdown in a murine model of laser-induced choroidal neovascularization.

Methods: Choroidal neovascularization was induced in the left eyes of 11 mice by infrared laser. Small interfering RNA (siRNA, 20 picomoles/10 μl) corresponding to PlGF mRNA was administered intravitreally by Hamilton syringe in all subjects. One month later, fluorescein angiography and histolologic examination were performed.

Results: No leakage was apparent in the 11 eyes treated with siRNA cognate to PlGF. The results of histological evaluation were consistent with angiographic findings showing absence of choroidal neovascularization.

Conclusion: Knockdown of the PlGF gene can inhibit the growth of laser-induced choroidal neovascularization in mice.

Keywords: Choroidal Neovascularization; Placental Growth Factor; Small Interfering RNA.