Matrix-assisted laser desorption ionization-time of flight mass spectrometry can accurately differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus (sensu stricto)

J Clin Microbiol. 2013 Sep;51(9):3113-6. doi: 10.1128/JCM.01239-13. Epub 2013 Jul 3.

Abstract

Among 36 Mycobacterium masilliense and 22 M. abscessus isolates identified by erm(41) PCR and sequencing analysis of rpoB and 23S rRNA genes, the rate of accurate differentiation between these two subspecies was 100% by cluster analysis of spectra generated by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Publication types

  • Evaluation Study

MeSH terms

  • Bacteriological Techniques / methods*
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA-Directed RNA Polymerases / genetics
  • Humans
  • Methyltransferases / genetics
  • Molecular Sequence Data
  • Nontuberculous Mycobacteria / chemistry*
  • Nontuberculous Mycobacteria / classification*
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 23S / genetics
  • Sensitivity and Specificity
  • Sequence Analysis, DNA
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • DNA, Bacterial
  • RNA, Ribosomal, 23S
  • Methyltransferases
  • DNA-Directed RNA Polymerases
  • RNA polymerase beta subunit

Associated data

  • GENBANK/CP003699
  • GENBANK/EU590128
  • GENBANK/GU143887
  • GENBANK/JF346872