Abstract
We found, unexpectedly, that the radical form of the carbon-centered quinone ketoxy radical adduct with a recently developed spin-trapping agent BMPO can not only be directly detected and identified using HPLC/high resolution MS, but can also be isolated and purified using semi-preparative HPLC, enabling direct observation of its clean 6-line ESR signal.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Carbon / chemistry*
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Chromatography, High Pressure Liquid
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Cyclic N-Oxides / chemistry*
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Electron Spin Resonance Spectroscopy
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Free Radicals / chemistry*
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Mass Spectrometry
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Quinones / chemistry*
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Spin Trapping
Substances
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5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide
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Cyclic N-Oxides
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Free Radicals
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Quinones
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Carbon