Detection of Candida albicans by using a designed fluorescence-quenched peptide

Wataru Aoki; Nao Kitahara; Aika Fujita; Seiji Shibasaki; Hironobu Morisaka; Kouichi Kuroda; Mitsuyoshi Ueda

doi:10.1016/j.jbiosc.2013.05.003

Detection of Candida albicans by using a designed fluorescence-quenched peptide

J Biosci Bioeng. 2013 Nov;116(5):573-5. doi: 10.1016/j.jbiosc.2013.05.003. Epub 2013 Jun 2.

Authors

Wataru Aoki¹, Nao Kitahara, Aika Fujita, Seiji Shibasaki, Hironobu Morisaka, Kouichi Kuroda, Mitsuyoshi Ueda

Affiliation

¹ The Japan Society for the Promotion of Science, Sakyo-ku, Kyoto 606-8502, Japan; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.

PMID: 23735329
DOI: 10.1016/j.jbiosc.2013.05.003

Abstract

Convenient detection of Candida albicans in human bodies is essential to treat patients with candidiasis. In this study, we designed a fluorescence-quenched peptide that emits fluorescence after being cleaved by virulent proteases secreted by C. albicans. The designed peptide will enable easy detection of candidiasis in immunocompromised patients.

Keywords: Candida albicans; Candidiasis; Diagnosis; Fluorescence-quenched peptide; Secreted aspartic protease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aspartic Acid Proteases / metabolism
Candida albicans / enzymology
Candida albicans / isolation & purification*
Candida albicans / pathogenicity
Candidiasis / diagnosis*
Candidiasis / microbiology*
Fluorescence*
Humans
Peptides / analysis*
Peptides / chemistry

Substances

Peptides
Aspartic Acid Proteases