Abstract
We found that Acinetobacter baumannii international clone II generally possesses unique GTA sequence at nucleotide positions 106-108 in the bla(OXA-51-like) genes. We exploited this to develop an easy and rapid method for discrimination of international clone II from other A. baumannii by employing pyrosequencing analyses of single nucleotide polymorphisms.
Keywords:
Acinetobacter baumannii; Pyrosequence; bla(OXA-51-like).
Copyright © 2013 Elsevier B.V. All rights reserved.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acinetobacter baumannii / classification
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Acinetobacter baumannii / enzymology
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Acinetobacter baumannii / genetics*
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Acinetobacter baumannii / isolation & purification
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Bacterial Typing Techniques / methods*
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Base Sequence
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High-Throughput Nucleotide Sequencing / methods*
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Molecular Sequence Data
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Phylogeny
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Polymorphism, Single Nucleotide*
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beta-Lactamases / genetics
Substances
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beta-Lactamases
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beta-lactamase OXA-51, Acinetobacter baumannii