Objective: To explore quantitative assessment indicators of Chrysanthemi Flos, and optimize the extraction process of Chrysanthemi Flos through orthogonal experimental design.
Method: The concentration of ethanol, amount of ethanol, extraction time and extraction frequency were selected as factors in the L9 (3(4)) orthogonal experiment. A comprehensive assessment was conducted with the peak area of the eight major common peaks in the fingerprint of Chrysanthemi Flos as the indicators.
Result: The optimum extraction process was selected as follows: using ultrasonic extraction method, adding 30-fold ethanol with 80% concentration, extracting for 2 times for extraction, 40 min for each time.
Conclusion: The optimized extraction process is reliable, with controllable assessment indicators, which is significant to the standardization of the extraction process and quality control of Chrysanthemi Flos preparations.