Fluorescent probes for live cell imaging of endogenous Guanine nitration

Chembiochem. 2013 Jun 17;14(9):1068-71. doi: 10.1002/cbic.201300129. Epub 2013 May 22.

Abstract

Seeing is believing: S-guanylation is a novel key mechanism by which signal transduction under oxidative stress is regulated. A chemical probe whose fluorescent intensity increases after the reaction with proteinous cysteine (S-guanylation) is described. The use of this probe revealed that S-guanylation products localized in lysosomes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Azides / chemistry
  • Cell Line
  • Coumarins / chemistry
  • Cysteine / chemistry
  • Fluorescent Dyes / chemistry*
  • Guanine / chemistry*
  • Guanine / metabolism
  • Humans
  • Immunohistochemistry
  • Lysosomes / metabolism
  • Mice
  • Microscopy, Fluorescence
  • Nitric Oxide / chemistry*

Substances

  • Azides
  • Coumarins
  • Fluorescent Dyes
  • Nitric Oxide
  • Guanine
  • Cysteine