Structure-based histidine substitution for optimizing pH-sensitive Staphylococcus protein A

J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Jun 15:929:155-60. doi: 10.1016/j.jchromb.2013.04.029. Epub 2013 Apr 26.

Abstract

Optimizing antibody purification is crucial to overcoming a bottleneck in the costly manufacturing process for antibody therapy. To address this issue, we designed a pH-sensitive Staphylococcus aureus protein A variant that retained its innate stability and affinity toward antibody. On the basis of structural information and mutation analysis data, we identified candidate positions for accumulative histidine substitutions to cause electrostatic repulsion under acidic conditions. The histidine substitutions effectively decreased the dissociation rate under acidic conditions by three orders of magnitude. Avoiding deleterious effects of the substitutions, we successfully engineered a protein A variant that exhibited high pH sensitivity and maintained affinity, thermal stability, and alkaline tolerance. The variant was capable of serving as an affinity ligand that made affinity chromatography under milder acidic conditions possible; the elution peak shifted from pH 4.2 to 5.6. Only two substitutions were needed to achieve this pH sensitivity. This structure-based approach is applicable to other protein-based ligands.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution
  • Chromatography, Affinity
  • Histidine / chemistry*
  • Hydrogen-Ion Concentration
  • Protein Engineering
  • Staphylococcal Protein A / chemistry*
  • Surface Plasmon Resonance

Substances

  • Staphylococcal Protein A
  • Histidine