Fabibacter pacificus sp. nov., a moderately halophilic bacterium isolated from seawater

Int J Syst Evol Microbiol. 2013 Oct;63(Pt 10):3710-3714. doi: 10.1099/ijs.0.051276-0. Epub 2013 Apr 26.

Abstract

A Gram-stain-negative, aerobic, moderately halophilic bacterium, strain DY53(T), was isolated from a deep-seawater sample collected from the eastern Pacific Ocean. This isolate grew in the presence of 0.5-10.0 % (w/v) NaCl, at pH 6.5-8.5 and at 15-40 °C. The optimum NaCl concentration for growth of DY53(T) was 2 % (w/v) at 35 °C. Chemotaxonomic analysis showed MK-7 as the predominant menaquinone and iso-C15 : 0, summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), iso-C15 : 1 G, iso-C15 : 0 3-OH and iso-C17 : 0 3-OH as major cellular fatty acids. The genomic DNA G+C content was 40.8 mol%. Phylogenetic trees based on 16S rRNA gene sequences revealed that Fabibacter halotolerans UST030701-097(T) was the closest neighbour, with 96.7 % sequence similarity. Based on phylogenetic, chemotaxonomic and phenotypic data, we propose that strain DY53(T) represents a novel species of the genus Fabibacter, for which the name Fabibacter pacificus sp. nov. is proposed. The type strain is DY53(T)( = CGMCC 1.12402(T) = JCM 18885(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis
  • Flavobacteriaceae / classification*
  • Flavobacteriaceae / genetics
  • Flavobacteriaceae / isolation & purification
  • Molecular Sequence Data
  • Pacific Ocean
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / analysis
  • Water Microbiology*

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • menaquinone 7

Associated data

  • GENBANK/KC005305