Epidermal growth factor receptor-containing exosomes induce tumor-specific regulatory T cells

Cancer Invest. 2013 Jun;31(5):330-5. doi: 10.3109/07357907.2013.789905. Epub 2013 Apr 24.

Abstract

The epidermal growth factor receptor (EGFR) has a close relation to lung cancer; its role in the pathogenesis of lung cancer is to be further elucidated. The results of this study indicate that about 80% exosomes purified from the LC biopsies contained EGFR; only about 2% exosomes contained EGFR in the samples from chronic lung inflammation. The purified exosomes induced tolerogenic DCs. Coculture of the tolerogenic DCs and Th0 cells generated the tumor antigen-specific regulatory T cells (Treg). The Tregs could suppress the tumor antigen specific CD8+ T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Neoplasm / immunology
  • Antigens, Neoplasm / metabolism
  • CD8-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / physiology
  • Carcinoma, Non-Small-Cell Lung / immunology
  • Carcinoma, Non-Small-Cell Lung / metabolism*
  • Carcinoma, Non-Small-Cell Lung / pathology
  • Case-Control Studies
  • Cell Proliferation
  • Cells, Cultured
  • Coculture Techniques
  • Dendritic Cells / immunology
  • ErbB Receptors / metabolism*
  • Exosomes / immunology
  • Exosomes / metabolism*
  • Humans
  • Indoleamine-Pyrrole 2,3,-Dioxygenase / metabolism
  • Lung Neoplasms / immunology
  • Lung Neoplasms / metabolism*
  • Lung Neoplasms / pathology
  • Pneumonia / metabolism
  • T-Lymphocytes, Regulatory / immunology*

Substances

  • Antigens, Neoplasm
  • Indoleamine-Pyrrole 2,3,-Dioxygenase
  • ErbB Receptors