Identification of sequence-related amplified polymorphism markers linked to the red leaf trait in ornamental kale (Brassica oleracea L. var. acephala)

Genet Mol Res. 2013 Apr 2;12(2):870-7. doi: 10.4238/2013.April.2.3.

Abstract

Artistic diversiform leaf color is an important agronomic trait that affects the market value of ornamental kale. In the present study, genetic analysis showed that a single-dominant gene, Re (red leaf), determines the red leaf trait in ornamental kale. An F2 population consisting of 500 individuals from the cross of a red leaf double-haploid line 'D05' with a white leaf double-haploid line 'D10' was analyzed for the red leaf trait. By combining bulked segregant analysis and sequence-related amplified polymorphism technology, we identified 3 markers linked to the Re/re locus. A genetic map of the Re locus was constructed using these sequence-related amplified polymorphism markers. Two of the markers, Me8Em4 and Me8Em17, were located on one side of Re/re at distances of 2.2 and 6.4 cM, whereas the other marker, Me9Em11, was located on the other side of Re/re at a distance of 3.7 cM. These markers could be helpful for the subsequent cloning of the red trait gene and marker-assisted selection in ornamental kale breeding programs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brassica / genetics*
  • Genes, Plant
  • Genetic Linkage
  • Genetic Markers*
  • Phenotype
  • Pigments, Biological / genetics*
  • Plant Leaves / genetics*
  • Polymorphism, Genetic*
  • Quantitative Trait Loci
  • Quantitative Trait, Heritable*
  • Random Amplified Polymorphic DNA Technique

Substances

  • Genetic Markers
  • Pigments, Biological