Effects of edaravone on the expression of β-defensin-2 mRNA in lung tissue of rats with myocardial ischemia reperfusion

Mol Med Rep. 2013 May;7(5):1683-7. doi: 10.3892/mmr.2013.1393. Epub 2013 Mar 22.

Abstract

The aim of this study was to investigate the effects of edaravone on lung injury caused by myocardial ischemia reperfusion (I/R) in rats. Wistar rats (n=24) were randomly divided into 4 groups: the sham operation (S group) and myocardial I/R groups (C group) and two edaravone‑treated groups (E1 and E2 groups). Rats in the E1 and E2 groups were injected with 3 or 10 mg/kg edaravone, respectively, 1 min before reperfusion. The rats were sacrificed and the lung tissue, bronchoalveolar lavage (BAL) fluid and serum were obtained. The concentration of serum creatine kinase isoenzyme (CK-MB) was determined, the lung permeability index (PPI) was calculated and β-defensin-2 (BD-2) mRNA expression in the lung tissue and BD-2 and TNF-α protein content levels were determined. Serum CK-MB activity and the PPI were increased, while BD-2 mRNA and BD‑2 and TNF-α protein levels in the lung tissue were upregulated in the C, E1 and E2 groups compared with the S group. The above‑mentioned indicators were decreased in the E1 and E2 groups compared with the IR group. The level of the decrease for indicators in the E2 group was significantly different compared with that in the E1 group. In conclusion, edaravone reduced the lung injury caused by myocardial I/R in rats. Its mechanism of action was not only oxygen free radical scavenging, but was also associated with a suppression of the inflammatory response of the lung tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antipyrine / analogs & derivatives*
  • Antipyrine / pharmacology
  • Blotting, Western
  • Creatine Kinase / blood
  • Edaravone
  • Gene Expression Regulation / drug effects*
  • Lung / drug effects
  • Lung / metabolism*
  • Lung / pathology
  • Lung / physiopathology
  • Male
  • Myocardial Reperfusion Injury / blood
  • Myocardial Reperfusion Injury / enzymology
  • Myocardial Reperfusion Injury / genetics*
  • Myocardial Reperfusion Injury / pathology*
  • Permeability / drug effects
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar
  • Tumor Necrosis Factor-alpha / metabolism
  • beta-Defensins / genetics*
  • beta-Defensins / metabolism

Substances

  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • beta-Defensins
  • Creatine Kinase
  • Edaravone
  • Antipyrine