Alveograph and Mixolab parameters associated with Puroindoline-D1 genes in Chinese winter wheats

J Sci Food Agric. 2013 Aug 15;93(10):2541-8. doi: 10.1002/jsfa.6073. Epub 2013 Mar 20.

Abstract

Background: Grain texture is one of the most important characteristics of bread wheat and has a significant influence on end-use qualities.

Results: Forty-three Chinese cultivars were tested under three environments and used to characterise kernel hardness, Puroindoline-D1 alleles and Alveograph and Mixolab parameters. The results indicated that SKCS hardness was positively correlated with Alveograph tenacity and P/L and Mixolab protein weakening (C2) and water absorption and negatively correlated with Mixolab starch gelatinisation (C3), amylasic activity (C4) and starch gelling (C5). Variance analysis showed that Puroindoline-D1 had a significant impact on SKCS hardness and most Alveograph and Mixolab parameters. Furthermore, among three Puroindoline-D1 genotypes, PINA-null/Pinb-D1a possessed the highest SKCS hardness, Alveograph tenacity and W and Mixolab stability and water absorption but the lowest Alveograph extensibility and G and Mixolab C3, C4 and C5. Pina-D1a/Pinb-D1a had the lowest SKCS hardness, Alveograph tenacity and W and Mixolab C2, water absorption and stability but the highest Alveograph extensibility and G and Mixolab C3, C4 and C5. Pina-D1a/Pinb-D1b possessed the lowest Mixolab C2 - C1, C3 - C2, C4 - C3 and C5 - C4.

Conclusion: Pina-D1a/Pinb-D1a was softer and had lower tenacity and water absorption. PINA-null/Pinb-D1a was harder and had higher tenacity and water absorption. Pina-D1a/Pinb-D1b had lower difference values among Mixolab parameters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amylases / metabolism
  • Bread*
  • China
  • Diet
  • Gels
  • Genes, Plant*
  • Genotype*
  • Hardness*
  • Humans
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Seeds / metabolism*
  • Starch / chemistry
  • Triticum / genetics*
  • Triticum / metabolism
  • Water

Substances

  • Gels
  • Plant Proteins
  • puroindoline protein, Triticum aestivum
  • Water
  • Starch
  • Amylases