CistromeFinder for ChIP-seq and DNase-seq data reuse

Bioinformatics. 2013 May 15;29(10):1352-4. doi: 10.1093/bioinformatics/btt135. Epub 2013 Mar 18.

Abstract

Summary: Chromatin immunoprecipitation and DNase I hypersensitivity assays with high-throughput sequencing have greatly accelerated the understanding of transcriptional and epigenetic regulation, although data reuse for the community of experimental biologists has been challenging. We created a data portal CistromeFinder that can help query, evaluate and visualize publicly available Chromatin immunoprecipitation and DNase I hypersensitivity assays with high-throughput sequencing data in human and mouse. The database currently contains 6378 samples over 4391 datasets, 313 factors and 102 cell lines or cell populations. Each dataset has gone through a consistent analysis and quality control pipeline; therefore, users could evaluate the overall quality of each dataset before examining binding sites near their genes of interest. CistromeFinder is integrated with UCSC genome browser for visualization, Primer3Plus for ChIP-qPCR primer design and CistromeMap for submitting newly available datasets. It also allows users to leave comments to facilitate data evaluation and update.

Availability: http://cistrome.org/finder.

Contact: xsliu@jimmy.harvard.edu or henry_long@dfci.harvard.edu.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Chromatin Immunoprecipitation*
  • Databases, Genetic*
  • Deoxyribonuclease I / metabolism
  • Epigenesis, Genetic
  • High-Throughput Nucleotide Sequencing*
  • Humans
  • Information Storage and Retrieval*
  • Mice
  • Oligonucleotide Array Sequence Analysis
  • Software

Substances

  • Deoxyribonuclease I