Preparation of recombinant atoxic form of exotoxin A from Pseudomonas aeruginosa

Bull Exp Biol Med. 2013 Jan;154(3):346-50. doi: 10.1007/s10517-013-1947-1.

Abstract

Nucleotide sequences encoding full-length protein of Pseudomonas aeruginosa exotoxin A and its atoxic form were cloned and expressed in Escherichia coli cells. Purified recombinant exotoxin and immune rabbit sera protected mice from exotoxin A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP Ribose Transferases / genetics
  • ADP Ribose Transferases / immunology*
  • ADP Ribose Transferases / toxicity
  • Animals
  • Bacterial Toxins / genetics
  • Bacterial Toxins / immunology*
  • Bacterial Toxins / toxicity
  • Cloning, Molecular
  • Exotoxins / genetics
  • Exotoxins / immunology*
  • Exotoxins / toxicity
  • Immune Sera
  • Immunization
  • Mice
  • Pseudomonas Infections / immunology*
  • Pseudomonas aeruginosa Exotoxin A
  • Pseudomonas aeruginosa* / genetics
  • Pseudomonas aeruginosa* / immunology
  • Pseudomonas aeruginosa* / metabolism
  • Rabbits
  • Recombinant Proteins / immunology
  • Recombinant Proteins / toxicity
  • Virulence Factors / genetics
  • Virulence Factors / immunology*
  • Virulence Factors / toxicity

Substances

  • Bacterial Toxins
  • Exotoxins
  • Immune Sera
  • Recombinant Proteins
  • Virulence Factors
  • ADP Ribose Transferases