Analysis of gene expression in skin using laser capture microdissection

Briana Lee; Mikhail Geyfman; Bogi Andersen; Xing Dai

doi:10.1007/978-1-62703-330-5_10

Analysis of gene expression in skin using laser capture microdissection

Methods Mol Biol. 2013:989:109-17. doi: 10.1007/978-1-62703-330-5_10.

Authors

Briana Lee¹, Mikhail Geyfman, Bogi Andersen, Xing Dai

Affiliation

¹ Department of Biological Chemistry, School of Medicine, University of California, Irvine, CA, USA.

PMID: 23483391
DOI: 10.1007/978-1-62703-330-5_10

Abstract

Gene expression analysis is a useful tool to study the molecular mechanisms underlying skin development and homeostasis. Here we describe a method that utilizes laser capture microdissection (LCM) to isolate RNAs from localized areas of skin, allowing the characterization of gene expression by RT-PCR and microarray technologies.

MeSH terms

Adult
Gene Expression / physiology*
Hair Follicle / cytology
Humans
Laser Capture Microdissection / methods*
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Skin / metabolism*

Abstract

MeSH terms

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