Abstract
Forty strains of Escherichia coli isolated from children under 5 years of age with acute diarreas, coming from different provinces of the country , were analyzed. Four important phenotypical determinants were tested: sorbosa, sorbitol, enterohemolysin and 0157:H7 serology, in order to select those strains from enterohemorrhagic or Shiga toxin-producing category. Likewise, they were characterized by biotyping and antimicrobial susceptibility methods. The use of phenotypical tests showed six strains with presumptive characteristics, four of which were most likely to be Shiga toxin-producing strains. In antimicrobial susceptibility test, the strains showed high resistance mainly to ampicillin and trimethrophin-sulfamethoxasole. Another interesting finding were intermediate resistance and susceptibility values to augmentin, aztreonan and ceftriaxone. There were 12 antimicrobial resistance patterns of which 10 were multi-resistant.
MeSH terms
-
Anti-Bacterial Agents / pharmacology
-
Bacterial Typing Techniques / methods*
-
Child, Preschool
-
Cuba / epidemiology
-
Diarrhea, Infantile / epidemiology
-
Diarrhea, Infantile / microbiology
-
Drug Resistance, Bacterial / genetics*
-
Drug Resistance, Multiple, Bacterial / genetics
-
Escherichia coli Infections / epidemiology
-
Escherichia coli Infections / microbiology
-
Escherichia coli O157 / drug effects
-
Escherichia coli O157 / genetics
-
Escherichia coli O157 / isolation & purification
-
Escherichia coli O157 / metabolism
-
Escherichia coli Proteins / analysis
-
Hemolysin Proteins / analysis
-
Humans
-
Infant
-
Microbial Sensitivity Tests
-
Phenotype
-
Serotyping
-
Shiga-Toxigenic Escherichia coli / classification*
-
Shiga-Toxigenic Escherichia coli / drug effects
-
Shiga-Toxigenic Escherichia coli / genetics
-
Shiga-Toxigenic Escherichia coli / isolation & purification
-
Shiga-Toxigenic Escherichia coli / metabolism
-
Sorbitol / metabolism
-
Sorbose / metabolism
Substances
-
Anti-Bacterial Agents
-
Escherichia coli Proteins
-
Hemolysin Proteins
-
enterohemolysin
-
Sorbitol
-
Sorbose