Modified Hodge test versus indirect carbapenemase test: prospective evaluation of a phenotypic assay for detection of Klebsiella pneumoniae carbapenemase (KPC) in Enterobacteriaceae

J Clin Microbiol. 2013 Apr;51(4):1291-3. doi: 10.1128/JCM.03240-12. Epub 2013 Feb 6.

Abstract

The currently recommended phenotypic test for the detection of carbapenemase-producing members of the family Enterobacteriaceae is the modified Hodge test (MHT). However, the MHT lacks specificity. Here we demonstrate an alternative phenotypic test, the indirect carbapenemase test, for the detection of blaKPC-producing isolates that has specificity superior to that of the MHT for non-Klebsiella Enterobacteriaceae.

Publication types

  • Evaluation Study

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / analysis*
  • Bacteriological Techniques / methods*
  • Enterobacteriaceae / drug effects*
  • Enterobacteriaceae / enzymology*
  • Humans
  • Prospective Studies
  • Sensitivity and Specificity
  • beta-Lactamases / analysis*
  • beta-Lactams / pharmacology*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • beta-Lactams
  • beta-Lactamases
  • carbapenemase