Small-molecule-mediated axonal branching in Caenorhabditis elegans

Chembiochem. 2013 Feb 11;14(3):307-10. doi: 10.1002/cbic.201200712. Epub 2013 Jan 29.

Abstract

An in vivo system for monitoring small-molecule-mediated neuronal branching has been developed by using C. elegans. Growth-promoting compounds can be detected by visual inspection of GFPlabeled cholinergic neurons, as axonal branching occurs following treatment with neurotrophic agents. Investigation of the structure-activity relationship of the neurotrophic natural product clovanemagnolol (1) led us to a comparable chemically edited derivative.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axons / drug effects
  • Axons / metabolism*
  • Caenorhabditis elegans / metabolism*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Phenols / chemistry
  • Phenols / pharmacology
  • Sesquiterpenes / chemistry
  • Sesquiterpenes / pharmacology
  • Small Molecule Libraries / chemistry*
  • Small Molecule Libraries / pharmacology
  • Structure-Activity Relationship

Substances

  • Phenols
  • Sesquiterpenes
  • Small Molecule Libraries
  • clovanemagnolol
  • Green Fluorescent Proteins