Formation of a cytosolic ganglioside-protein complex following administration of photoreactive ganglioside GM1 to human fibroblasts in culture

V Chigorno; M Valsecchi; D Acquotti; S Sonnino; G Tettamanti

doi:10.1016/0014-5793(90)81406-e

Formation of a cytosolic ganglioside-protein complex following administration of photoreactive ganglioside GM1 to human fibroblasts in culture

FEBS Lett. 1990 Apr 24;263(2):329-31. doi: 10.1016/0014-5793(90)81406-e.

Authors

V Chigorno¹, M Valsecchi, D Acquotti, S Sonnino, G Tettamanti

Affiliation

¹ Department of Medical Chemistry and Biochemistry, Medical School, University of Milan, Italy.

PMID: 2335236
DOI: 10.1016/0014-5793(90)81406-e

Abstract

A GM 1 ganglioside derivative bearing a photoreactive nitrophenyl azide group and tritium labeled at the acetyl group of N-acetylneuraminic acid, has been administered to cultured human fibroblasts. With photolabeling experiments we found that a portion of the ganglioside in the cell cytosol was associated with a soluble protein of about 30 kDa.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cells, Cultured
Cytosol / metabolism*
Electrophoresis, Polyacrylamide Gel
Fibroblasts / metabolism*
G(M1) Ganglioside / metabolism*
Humans
Molecular Weight
Photochemistry
Protein Binding

Substances

G(M1) Ganglioside