A novel two dimensional surface plasmon resonance (SPR) sensor system with a multi-point sensing region is described. The use of multiplied beam splitting optics, as a core technology, permitted multi-point sensing to be achieved. This system was capable of simultaneously measuring nine sensing points. Calibration curves for sucrose obtained on nine sensing points were linear in the range of 0-10% with a correlation factor of 0.996-0.998 with a relative standard deviation of 0.090-4.0%. The detection limits defined as S/N = 3 were 1.98 × 10(-6) - 3.91 × 10(-5) RIU. This sensitivity is comparable to that of conventional SPR sensors.