Longitudinal strain impairment as a marker of the progression of heart failure with preserved ejection fraction in a rat model

J Am Soc Echocardiogr. 2013 Mar;26(3):316-23. doi: 10.1016/j.echo.2012.11.015. Epub 2013 Jan 6.

Abstract

Background: Recent advances in very high frame rate use of ultrasonography have enabled the application of two-dimensional speckle-tracking echocardiography (STE) to small animal cardiac functional assessments. In this study, two-dimensional STE was applied to a rat model of hypertensive heart failure with preserved ejection fraction to clarify consequences of left ventricular (LV) wall deformation in the progression of heart failure with preserved ejection fraction.

Methods: STE was performed every 2 weeks in Dahl salt-sensitive rats fed a 0.3% (control group) or 8% (hypertension [HT] group) sodium chloride diet from 6 to 14 weeks of age. Longitudinal, radial, and circumferential global strain and strain rate were measured, and the time courses of these parameters were observed.

Results: Deterioration of longitudinal strain occurred in the early phase of the progression of LV hypertrophy and continued to worsen until congestive heart failure developed (longitudinal strain in the HT group: 25 ± 3% at 10 weeks, 21 ± 4% at 12 weeks, and 18 ± 2% at 14 weeks; longitudinal strain in controls was preserved during the experimental period). At 12 weeks, radial strain (HT group, 35 ± 7%; controls, 41 ± 10%) had deteriorated at the late stage of manifest diastolic dysfunction. Throughout the experiments, circumferential strain was preserved (HT group, 35 ± 6%; control group, 35 ± 5%), and no significant increase in short-axis function was observed.

Conclusions: STE is applicable to the small animal heart and detected LV wall long-axis dysfunction preceding short-axis dysfunction or overt congestive heart failure in the progression of hypertensive LV hypertrophy in a rat model.

MeSH terms

  • Analysis of Variance
  • Animals
  • Disease Models, Animal
  • Disease Progression
  • Echocardiography / methods*
  • Heart Failure / diagnostic imaging*
  • Heart Failure / physiopathology*
  • Rats
  • Rats, Inbred Dahl
  • Reproducibility of Results
  • Stroke Volume