[Citogenetic and molecular genetic studies in infertility in eastern Hungary]

Orv Hetil. 2013 Jan 13;154(2):52-61. doi: 10.1556/OH.2013.29510.
[Article in Hungarian]

Abstract

Introduction: In developed countries 10-15% of the couples are affected by infertility. In half of them genetic factors can be identified.

Aims: We studied genetic alterations in infertility in Hungarian patients.

Methods: Cyogenetic analyses were performed in 195 females and 305 males. In 17 females FMR1 mutations, in 150 males Y microdeletions, and aneuploidy were studied in the sperm of 28 males. In a carrier male sperm meiotic segregation was studied.

Results: The most common aberrations in females were X chromosome aneuploidia and inversion (3.6%), while the same in males Klinefelter-syndrome (3.3%) and autosomal translocations (2%). In two females FMR1 premutation was found. While Y microdeletions were identified only in azoospermic and severe oligozoospermic men, partial microdeletions could also be detected in normozoospermic males. A higher aberration rate was found in cases with abnormality in both the number and motility of sperm. In a male patient with 46,XY,t(3;6)(q21;q23) karyotype, 53.2% of spem carried unbalanced chromosome assortment.

Conclusions: Knowledge of abnormalities may help in genetic counseling and choosing the most effective reproduction technique.

Publication types

  • English Abstract

MeSH terms

  • Adult
  • Aneuploidy*
  • Azoospermia / genetics
  • Chromosomes, Human, X / genetics*
  • Cytogenetics / methods
  • Female
  • Genetic Counseling
  • Humans
  • Hungary
  • Hyaluronic Acid / metabolism
  • In Situ Hybridization, Fluorescence
  • Infertility / genetics*
  • Infertility, Male / genetics
  • Karyotyping
  • Klinefelter Syndrome / genetics
  • Male
  • Molecular Biology / methods
  • Mutation*
  • Oligospermia / genetics
  • Sex Chromosome Aberrations*
  • Sex Chromosome Disorders / genetics*
  • Sex Chromosome Disorders of Sex Development / genetics
  • Spermatozoa / metabolism
  • Translocation, Genetic

Substances

  • Hyaluronic Acid