Transcriptional, biochemical and histochemical investigation on laccase expression during Tuber melanosporum Vittad. development

Osvaldo Zarivi; Antonella Bonfigli; Sabrina Colafarina; Pierpaolo Aimola; Anna Maria Ragnelli; Michele Miranda; Giovanni Pacioni

doi:10.1016/j.phytochem.2012.11.019

Transcriptional, biochemical and histochemical investigation on laccase expression during Tuber melanosporum Vittad. development

Phytochemistry. 2013 Mar:87:23-9. doi: 10.1016/j.phytochem.2012.11.019. Epub 2012 Dec 29.

Authors

Osvaldo Zarivi¹, Antonella Bonfigli, Sabrina Colafarina, Pierpaolo Aimola, Anna Maria Ragnelli, Michele Miranda, Giovanni Pacioni

Affiliation

¹ Department of Life, Health & Environmental Sciences, University of L'Aquila, I 67010 Coppito-L'Aquila, Italy.

PMID: 23276677
DOI: 10.1016/j.phytochem.2012.11.019

Abstract

The cDNAs of Tuber melanosporum laccases (Tmellcc1 and Tmellcc2) have been cloned. From the cloned cDNAs probes were prepared to investigate the expression levels of the Tmellcc1 and Tmellcc2 genes in the free living mycelium (FLM), ectomycorrhizae (ECM) and different developmental stages of fruit body (FB) by quantitative PCR (qPCR). The mRNA expression levels agree with the changes of laccase activities. The histochemical data agree with the qPCR and biochemical results. The highest laccase expression occurs in the ECM, when the host plant roots are invaded by the fungal mycelium.

MeSH terms

Ascomycota / enzymology*
Ascomycota / pathogenicity
Corylus / microbiology*
Laccase / genetics
Laccase / metabolism*
Mycelium / enzymology
Mycelium / pathogenicity
Plant Roots / microbiology*

Substances

Laccase