Curcumin derivatives promote Schwann cell differentiation and improve neuropathy in R98C CMT1B mice

Brain. 2012 Dec;135(Pt 12):3551-66. doi: 10.1093/brain/aws299.

Abstract

Charcot-Marie-Tooth disease type 1B is caused by mutations in myelin protein zero. R98C mice, an authentic model of early onset Charcot-Marie-Tooth disease type 1B, develop neuropathy in part because the misfolded mutant myelin protein zero is retained in the endoplasmic reticulum where it activates the unfolded protein response. Because oral curcumin, a component of the spice turmeric, has been shown to relieve endoplasmic reticulum stress and decrease the activation of the unfolded protein response, we treated R98C mutant mice with daily gastric lavage of curcumin or curcumin derivatives starting at 4 days of age and analysed them for clinical disability, electrophysiological parameters and peripheral nerve morphology. Heterozygous R98C mice treated with curcumin dissolved in sesame oil or phosphatidylcholine curcumin performed as well as wild-type littermates on a rotarod test and had increased numbers of large-diameter axons in their sciatic nerves. Treatment with the latter two compounds also increased compound muscle action potential amplitudes and the innervation of neuromuscular junctions in both heterozygous and homozygous R98C animals, but it did not improve nerve conduction velocity, myelin thickness, G-ratios or myelin period. The expression of c-Jun and suppressed cAMP-inducible POU (SCIP)-transcription factors that inhibit myelination when overexpressed-was also decreased by treatment. Consistent with its role in reducing endoplasmic reticulum stress, treatment with curcumin dissolved in sesame oil or phosphatidylcholine curcumin was associated with decreased X-box binding protein (XBP1) splicing. Taken together, these data demonstrate that treatment with curcumin dissolved in sesame oil or phosphatidylcholine curcumin improves the peripheral neuropathy of R98C mice by alleviating endoplasmic reticulum stress, by reducing the activation of unfolded protein response and by promoting Schwann cell differentiation.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Action Potentials / drug effects
  • Action Potentials / genetics
  • Age Factors
  • Analysis of Variance
  • Animals
  • Animals, Newborn
  • Anti-Inflammatory Agents, Non-Steroidal / therapeutic use*
  • Arginine / genetics
  • COS Cells / drug effects
  • Cell Differentiation / drug effects*
  • Cells, Cultured
  • Charcot-Marie-Tooth Disease* / drug therapy
  • Charcot-Marie-Tooth Disease* / genetics
  • Charcot-Marie-Tooth Disease* / pathology
  • Chlorocebus aethiops
  • Curcumin / therapeutic use*
  • Cysteine / genetics
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Disease Models, Animal
  • Early Growth Response Protein 2 / metabolism
  • Electric Stimulation / methods
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / genetics
  • Green Fluorescent Proteins / genetics
  • Humans
  • Mice
  • Mice, Transgenic
  • Motor Activity / drug effects
  • Motor Activity / genetics
  • Muscle Strength / drug effects
  • Muscle Strength / genetics
  • Mutation / genetics
  • Myelin P0 Protein / genetics*
  • Myelin P0 Protein / metabolism
  • Neuromuscular Junction / drug effects
  • Neuromuscular Junction / genetics
  • Octamer Transcription Factor-6 / metabolism
  • Protein Folding / drug effects
  • Proto-Oncogene Proteins c-jun / metabolism
  • Regulatory Factor X Transcription Factors
  • Rotarod Performance Test
  • Schwann Cells / drug effects*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transfection
  • X-Box Binding Protein 1

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • DNA-Binding Proteins
  • Early Growth Response Protein 2
  • Egr2 protein, mouse
  • MPZ protein, human
  • Myelin P0 Protein
  • Proto-Oncogene Proteins c-jun
  • Regulatory Factor X Transcription Factors
  • Transcription Factors
  • X-Box Binding Protein 1
  • XBP1 protein, human
  • Xbp1 protein, mouse
  • enhanced green fluorescent protein
  • Octamer Transcription Factor-6
  • Green Fluorescent Proteins
  • Arginine
  • Curcumin
  • Cysteine