Decapitation-induced changes in inositol phosphates in rat brain

Biochem Biophys Res Commun. 1990 Mar 30;167(3):1294-301. doi: 10.1016/0006-291x(90)90664-9.

Abstract

Decapitation resulted in a time-dependent production of inositol phosphates in rat brain. This production was analyzed by measuring both the radioactivity and the concentrations of inositol phosphates generated from [3H]inositol-labeled phospholipids. Both measurements produced the same time-dependent changes, including a rapid decrease in inositol 1,4,5-trisphosphate within 1.5 min, a 6-fold increase in inositol 1,4-bisphosphate to a maximum at 1.5 min, a 5-fold rise in inositol 4-monophosphate to a maximum at 2.5 min, and little change in inositol 1-monophosphate. The temporal changes in the mass and radioactivity of these compounds, together with the decrease in labeling of phosphatidylinositol 4,5-bisphosphates, support the idea that the inositol phosphates originate from the hydrolysis of phosphatidylinositol 4,5-bisphosphates and not from either the direct hydrolysis of phosphatidylinositol 4-phosphates or phosphatidylinositols.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Brain / metabolism*
  • Brain Ischemia / metabolism*
  • Cerebral Cortex / metabolism
  • Decerebrate State
  • Inositol / metabolism
  • Inositol Phosphates / isolation & purification
  • Inositol Phosphates / metabolism*
  • Kinetics
  • Postmortem Changes
  • Rats
  • Rats, Inbred Strains
  • Time Factors

Substances

  • Inositol Phosphates
  • Inositol