Association between HBX status, aflatoxin-induced R249S TP53 mutation and risk of hepatocellular carcinoma in a case-control study from Thailand

Cancer Lett. 2013 Apr 30;331(1):46-51. doi: 10.1016/j.canlet.2012.11.012. Epub 2012 Nov 27.

Abstract

Hepatocellular carcinoma (HCC) is associated with hepatitis B virus (HBV) chronicity and dietary exposure to aflatoxin, a mutagen targeting codon 249 of tumor suppressor TP53 (R249S mutation). Based on a case-control in Thailand, we have measured R249S and the status of HBX gene in plasma DNA of 176 cases and 133 referents. Detection of HBX complete sequences was associated with R249S in HCC with no documented prior cirrhosis but not in HCC developing in a context of cirrhosis or in non-cancer chronic liver diseases. Thus, R249S may specifically cooperate with HBX in a pathway to HCC that bypasses cirrhosis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aflatoxins / adverse effects*
  • Aged
  • Biomarkers / blood*
  • Carcinoma, Hepatocellular / blood
  • Carcinoma, Hepatocellular / etiology*
  • Carcinoma, Hepatocellular / pathology
  • Case-Control Studies
  • DNA / analysis
  • DNA / genetics
  • Female
  • Hepatitis B / blood
  • Hepatitis B / complications*
  • Hepatitis B / virology
  • Hepatitis B virus / genetics
  • Humans
  • Liver Cirrhosis / blood
  • Liver Cirrhosis / complications*
  • Liver Cirrhosis / pathology
  • Liver Neoplasms / blood
  • Liver Neoplasms / etiology
  • Liver Neoplasms / pathology
  • Male
  • Middle Aged
  • Mutation / genetics*
  • Poisons / adverse effects
  • Polymerase Chain Reaction
  • Risk Factors
  • Trans-Activators / blood*
  • Tumor Suppressor Protein p53 / genetics*
  • Viral Regulatory and Accessory Proteins

Substances

  • Aflatoxins
  • Biomarkers
  • Poisons
  • TP53 protein, human
  • Trans-Activators
  • Tumor Suppressor Protein p53
  • Viral Regulatory and Accessory Proteins
  • hepatitis B virus X protein
  • DNA