Purification of antigen B from Taenia solium cysticerci by affinity to mammalian collagen

J P Laclette; A Alagón; K Willms; A Torre-Blanco

Purification of antigen B from Taenia solium cysticerci by affinity to mammalian collagen

J Parasitol. 1990 Apr;76(2):273-5.

Authors

J P Laclette¹, A Alagón, K Willms, A Torre-Blanco

Affiliation

¹ Department of Immunology, Instituto de Investigaciones Biomédicas, U.N.A.M., México, D.F.

PMID: 2319431

Abstract

A 1-step procedure for the easy and rapid purification of milligram amounts of antigen B from a crude extract of Taenia solium cysticerci is described here. This procedure takes advantage of the property of the antigen B to bind to collagen and is based on antigen adsorption to polymeric collagen.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Animals
Antigens, Helminth / isolation & purification*
Blotting, Western
Chromatography, Affinity
Collagen / metabolism*
Cysticercus / immunology*
Electrophoresis, Polyacrylamide Gel
Swine
Taenia / immunology*

Substances

Antigens, Helminth
Collagen